Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7L parental
cell types
immortalized breast epithelial cell line
chip antibody
ERα
agent
treated with E2
time point
24 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA extracted using Quick-RNA MiniPrep Kit from ZYMO Research TCC was performed as described (Kalhor et al. 2012). ChIP-seq and RNA-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
36851361
Reads aligned (%)
88.6
Duplicates removed (%)
8.7
Number of peaks
4398 (qval < 1E-05)

hg38

Number of total reads
36851361
Reads aligned (%)
91.0
Duplicates removed (%)
7.1
Number of peaks
4750 (qval < 1E-05)

Base call quality data from DBCLS SRA