Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CHD7

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
hiPSC
tissue
hiPSC
genotype/variation
wild type
chip-antibody
CHD7 (CST, #6505, clone D3F5)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For single fixation with formaldehyde, cells were fixed with 1% formaldehyde-containing medium for 10 min. For double fixation, the cells were first fixed with 2 mM EGS for 30 min followed by with 1% formaldehyde-containing medium for 10 min. Libraries were prepared using KAPA Hyperprep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
39985011
Reads aligned (%)
52.2
Duplicates removed (%)
46.6
Number of peaks
20328 (qval < 1E-05)

hg38

Number of total reads
39985011
Reads aligned (%)
53.7
Duplicates removed (%)
45.5
Number of peaks
20355 (qval < 1E-05)

Base call quality data from DBCLS SRA