GSM2891676: prpa HeLa IP 2 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RPA2
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
ChIP-Seq of pRPA in HeLa cells
cell line
HeLa
genotype/variation
WT
treatment
untreated
antibody
Bethyl Laboratories #A-300246A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed in the sonication buffer (50mM Hepes pH8, 140mM NaCl, 1mM EDTA, 1% Triton, 0,1% sodium deoxycholate, 0.5% SDS). Chromatin was treated with protease K followed by Phenol/chloroform extraction and ethanol precipitation. NeoPrep Illumina system for automatic NGS library preparation using TruSeq Nano DNA Library Prep Kit for NeoPrep (Illumina)_Sequencer:HiSeq 4000 (1x51 nt).