Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RPA2

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
ChIP-Seq of pRPA in shASF cells
cell line
HeLa
genotype/variation
shASF
treatment
untreated
antibody
Bethyl Laboratories #A-300246A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed in the sonication buffer (50mM Hepes pH8, 140mM NaCl, 1mM EDTA, 1% Triton, 0,1% sodium deoxycholate, 0.5% SDS). Chromatin was treated with protease K followed by Phenol/chloroform extraction and ethanol precipitation. NeoPrep Illumina system for automatic NGS library preparation using TruSeq Nano DNA Library Prep Kit for NeoPrep (Illumina)_Sequencer:HiSeq 4000 (1x51 nt).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
56773551
Reads aligned (%)
85.6
Duplicates removed (%)
7.2
Number of peaks
1424 (qval < 1E-05)

hg38

Number of total reads
56773551
Reads aligned (%)
87.6
Duplicates removed (%)
5.8
Number of peaks
1577 (qval < 1E-05)

Base call quality data from DBCLS SRA