Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TEAD1

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
TEAD1-ChIPSeq-K3AKO-SF
cell type
HCT116
genotype/variation
KDM3A knockout
culture condition
SF; addition of FBS into the no-serum culture after cell-starved for for 24 h
chip antibody
TEAD1(Cell Signaling Technology.12292S)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated and enzyme digestion nuclei and histone-DNA complexes were isolated with antibody. Vazyme ND604 kit was used for ChIPSeq library construction. Brifely, about 10-100ng dsDNA was used as initiation for end repair and prepared for adaptor ligation. After adaptor ligation, PCR amplification with 8-13 cycles for each library and gel selection was used for library size selection and purification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16829461
Reads aligned (%)
123.0
Duplicates removed (%)
1.5
Number of peaks
764 (qval < 1E-05)

hg38

Number of total reads
16829461
Reads aligned (%)
124.0
Duplicates removed (%)
1.4
Number of peaks
814 (qval < 1E-05)

Base call quality data from DBCLS SRA