GSM2915992: REP2-TEAD1-ChIPSeq-WT-S; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
TEAD1(Cell Signaling Technology.12292S)
Sequenced DNA Library
Lysates were clarified from sonicated and enzyme digestion nuclei and histone-DNA complexes were isolated with antibody. Vazyme ND604 kit was used for ChIPSeq library construction. Brifely, about 10-100ng dsDNA was used as initiation for end repair and prepared for adaptor ligation. After adaptor ligation, PCR amplification with 8-13 cycles for each library and gel selection was used for library size selection and purification.