Antigen

Antigen Class

TFs and others

Antigen

TEAD1

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

TEAD1-ChIPSeq-WT-S

cell type

HCT116

genotype/variation

wild type

culture condition

starvation; no-serum

chip antibody

TEAD1(Cell Signaling Technology.12292S)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated and enzyme digestion nuclei and histone-DNA complexes were isolated with antibody. Vazyme ND604 kit was used for ChIPSeq library construction. Brifely, about 10-100ng dsDNA was used as initiation for end repair and prepared for adaptor ligation. After adaptor ligation, PCR amplification with 8-13 cycles for each library and gel selection was used for library size selection and purification.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

26169673

Reads aligned (%)

56.3

Duplicates removed (%)

1.6

Number of peaks

869 (qval < 1E-05)

hg38

Number of total reads

26169673

Reads aligned (%)

56.7

Duplicates removed (%)

1.5

Number of peaks

946 (qval < 1E-05)