Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-134-VI
Primary Tissue
Breast
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Mammary epithelial cells
cell type
invasive lobular carcinoma cells
cell line
MDA-MB-134
cell passage
40-50
chip antibody
ERα (Santa Cruz, HC-20)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Input DNA was purified from cell lysate and ER-DNA complexes were isolated by the corresponding antibody. DNA samples were sent to Genome Quebec Innovation Center (McGill University, Montreal, Quebec, Canada) for next generation sequencing. Libraries were prepared according to Illumina TruSeq ChIP Sample Prep Kit (#IP-202-1012).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
97710371
Reads aligned (%)
85.7
Duplicates removed (%)
11.5
Number of peaks
1815 (qval < 1E-05)

hg38

Number of total reads
97710371
Reads aligned (%)
88.3
Duplicates removed (%)
9.7
Number of peaks
1983 (qval < 1E-05)

Base call quality data from DBCLS SRA