Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPSC derived hematopoietic cells
NA
NA

Attributes by original data submitter

Sample

source_name
HPC-5F
cell line
IPSC-derived Hematopoietic Progenitor Cells (transduced with lentivirus)
overexpression
Control
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Antibody epitope: H3K4me2. Vendor: Active Motif. Catalog number: 39141. Lot: #01008001. Antibody epitope: V5. Vendor: MBL intl. Catalog number: M167-3. Lot: #5. ChIP-seq Illumina libraries were prepared and analyzed as previously described (Mikkelsen et al., 2010).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
7012671
Reads aligned (%)
98.0
Duplicates removed (%)
1.9
Number of peaks
217 (qval < 1E-05)

hg19

Number of total reads
7012671
Reads aligned (%)
97.1
Duplicates removed (%)
2.0
Number of peaks
193 (qval < 1E-05)

Base call quality data from DBCLS SRA