Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

HPC-5F

cell line

HPC-5F

overexpression

V5-tagged HMGA2-S

chip antibody

V5

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Antibody epitope: H3K4me2. Vendor: Active Motif. Catalog number: 39141. Lot: #01008001. Antibody epitope: V5. Vendor: MBL intl. Catalog number: M167-3. Lot: #5. ChIP-seq Illumina libraries were prepared and analyzed as previously described (Mikkelsen et al., 2010).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

69752734

Reads aligned (%)

97.8

Duplicates removed (%)

27.4

Number of peaks

1011 (qval < 1E-05)

hg19

Number of total reads

69752734

Reads aligned (%)

96.9

Duplicates removed (%)

27.8

Number of peaks

562 (qval < 1E-05)