Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CREB1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-134-VI
Primary Tissue
Breast
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
pCREB1 FI Chip-seq
cell line
MDA-MB-134
cell type
Epithelial ERalpha positive breast cancer cell line
passage
between 10 and 12
treated with
a cocktail of 10 µM forskolin plus 100 µM 3-isobutyl-1-methylxanthine (FI) for 1hr
chip antibodies
pCREB1 (Cell Signalling Technology, 87G3, lot 14)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were processed for ChIP after crosslinking with 1% formaldehyde according to the protocols of Schmidt et al. (2009) Methods 48, 240-248. The following antibodies were used: the rabbit monoclonal antibody 87G3 against phospho-CREB from Cell Signaling Technology and the rabbit polyclonal A300-498A against ERα from Bethyl Laboratories. ChIP samples were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
25557587
Reads aligned (%)
52.0
Duplicates removed (%)
45.4
Number of peaks
4532 (qval < 1E-05)

hg38

Number of total reads
25557587
Reads aligned (%)
53.9
Duplicates removed (%)
44.0
Number of peaks
4813 (qval < 1E-05)

Base call quality data from DBCLS SRA