Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CREB1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-134-VI
Primary Tissue
Breast
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
pCREB1 DMSO ChIP-seq
cell line
MDA-MB-134
cell type
Epithelial ERalpha positive breast cancer cell line
passage
between 10 and 12
treated with
DMSO
chip antibodies
pCREB1 (Cell Signalling Technology, 87G3, lot 14)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were processed for ChIP after crosslinking with 1% formaldehyde according to the protocols of Schmidt et al. (2009) Methods 48, 240-248. The following antibodies were used: the rabbit monoclonal antibody 87G3 against phospho-CREB from Cell Signaling Technology and the rabbit polyclonal A300-498A against ERα from Bethyl Laboratories. ChIP samples were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
28290490
Reads aligned (%)
62.8
Duplicates removed (%)
28.5
Number of peaks
1373 (qval < 1E-05)

hg38

Number of total reads
28290490
Reads aligned (%)
64.2
Duplicates removed (%)
27.7
Number of peaks
1479 (qval < 1E-05)

Base call quality data from DBCLS SRA