Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Kidney
Cell type
Kidney
MeSH Description
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.

Attributes by original data submitter

Sample

source_name
kidney tissue
strain
C57BL/6J
genotype
Wild-type
treatment
BD
tissue
kidney

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Precipitation was performed with 1µg of an antibody against acetylated histone 3 lysine 27 (anti-H3K27ac, Abcam, ab4729), following the steps outlined in Ihn et al. DNA was isolated following the IP, and libraries were prepared using the Illumina TruSeq ChIP protocol TruSeq libraries were constructed as per manufacturer's recommendations.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
67843180
Reads aligned (%)
98.8
Duplicates removed (%)
43.3
Number of peaks
10098 (qval < 1E-05)

mm9

Number of total reads
67843180
Reads aligned (%)
98.6
Duplicates removed (%)
43.3
Number of peaks
10077 (qval < 1E-05)

Base call quality data from DBCLS SRA