Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MDA-MB-231-ERb_Input_Full_24_hrs_Dox_3_hrs_1nM_Estradiol

cell line

MDA-MB-231-ER-beta

cell type

Triple Negative Breast Cancer Cell Line

passages

20-25

treated with

1 nM estradiol for 3 h

chip antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear lysates were prepared and ER-beta bound chromatin was purified using the Flag-specific antibody. DNA was prepared for Illumina sequencing on the HiSeq 2500 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

23294064

Reads aligned (%)

31.2

Duplicates removed (%)

1.3

Number of peaks

250 (qval < 1E-05)

hg19

Number of total reads

23294064

Reads aligned (%)

30.9

Duplicates removed (%)

2.3

Number of peaks

448 (qval < 1E-05)