GSM2918262: jc2831 MDA-MB-231-ERb ERb Full 24 hrs Dox 3 hrs ethanol; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-231-ERb_ERb_Full_24_hrs_Dox_3_hrs_ethanol
cell line
MDA-MB-231-ER-beta
cell type
Triple Negative Breast Cancer Cell Line
passages
20-25
treated with
ethanol vehicle for 3 h
chip antibody
Flag (Sigma, M2)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were prepared and ER-beta bound chromatin was purified using the Flag-specific antibody. DNA was prepared for Illumina sequencing on the HiSeq 2500 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.