Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Bone marrow nuclear cells

NA

NA

Sample

source_name

AML patient 3 bone marrow nuclear cells

tissue

Bone marrow

chip antibody

H3K27me3 (Millipore, Cat. 07-449, Lot 2194165)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were precipitated with antibody. ChIP-seq library preparation was performed using TruSeqChIP Sample Prep Kit (Illumina) according to the manufacturer's instructions.10ng of input ChIP-enriched DNA was used for ChIP-seq library preparation. Final libraries were checked using High Sensitivity chips in 2100 Bioanalyzer (Agilent). Average fragment size of final libraries was found to be 280 ± 8bp. Paired-end sequencing (2 x 100 bp) of these libraries were performed in HiSeq-2500 (Illumina) according to the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

50893463

Reads aligned (%)

97.7

Duplicates removed (%)

67.6

Number of peaks

1156 (qval < 1E-05)

hg19

Number of total reads

50893463

Reads aligned (%)

96.5

Duplicates removed (%)

68.5

Number of peaks

588 (qval < 1E-05)