Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
breast cancer cells
cell type
MCF-7 cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were lysed and samples were sonicated to fragment chromatin. Libraries were prepared according to Roche's instructions. KAPA DNA HyperPrep Library Kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
34866830
Reads aligned (%)
96.4
Duplicates removed (%)
1.4
Number of peaks
980 (qval < 1E-05)

hg19

Number of total reads
34866830
Reads aligned (%)
95.5
Duplicates removed (%)
1.6
Number of peaks
463 (qval < 1E-05)

Base call quality data from DBCLS SRA