Antigen

Antigen Class

TFs and others

Antigen

SUMO2

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

breast cancer cells

cell type

MCF-7 cells

chip antibody

SUMO2/3 (Cedarlane M114-3)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were lysed. SUMO2/3-DNA complexes were isolated with the appropriate antibody. Libraries were prepared according to Roche's instructions. KAPA DNA HyperPrep Library Kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

37642398

Reads aligned (%)

88.5

Duplicates removed (%)

2.6

Number of peaks

1628 (qval < 1E-05)

hg19

Number of total reads

37642398

Reads aligned (%)

87.6

Duplicates removed (%)

2.8

Number of peaks

776 (qval < 1E-05)