Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
breast cancer cells
cell type
MCF-7 cells
chip antibody
ERα HC-20 (Santa Cruz Biotechnology sc-543)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were lysed. ERα-DNA complexes complexes were isolated with the appropriate antibody. Libraries were prepared according to Roche's instructions. KAPA DNA HyperPrep Library Kit.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
25887922
Reads aligned (%)
124.1
Duplicates removed (%)
22.5
Number of peaks
449 (qval < 1E-05)

hg38

Number of total reads
25887922
Reads aligned (%)
125.8
Duplicates removed (%)
22.1
Number of peaks
668 (qval < 1E-05)

Base call quality data from DBCLS SRA