Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

breast cancer cells

cell type

MCF-7 cells

chip antibody

ERα HC-20 (Santa Cruz Biotechnology sc-543)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were lysed. ERα-DNA complexes complexes were isolated with the appropriate antibody. Libraries were prepared according to Roche's instructions. KAPA DNA HyperPrep Library Kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

40032563

Reads aligned (%)

60.4

Duplicates removed (%)

4.4

Number of peaks

3331 (qval < 1E-05)

hg19

Number of total reads

40032563

Reads aligned (%)

60.0

Duplicates removed (%)

4.5

Number of peaks

3108 (qval < 1E-05)