Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NRF1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
tissue
cervix adenocarcinoma
chip antibody
anti-NRF1 antibody (Cell Signaling, D5B10)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were cross-linked with 1.5 mM EGS (Thermo Scientific) for 20 min, followed by treatment with 1% formaldehyde for 10 min; the samples were then lysed and digested with micrococcal nuclease (New England Biolabs) to shear DNA.ChIP was performed with anti-NRF1 antibody (Cell Signaling, D5B10).The antibody incubation were pereformed overnight at 4℃. The DNA libraries were prepared from 1 ng of ChIP and input samples quantified with Qubit Fluorometer (Life Technologies), using Mondrian SP+ and Ovation SP Ultralow DR Multiplex System (TaKaRa).braries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
25899159
Reads aligned (%)
89.6
Duplicates removed (%)
7.7
Number of peaks
1295 (qval < 1E-05)

hg38

Number of total reads
25899159
Reads aligned (%)
90.4
Duplicates removed (%)
7.2
Number of peaks
1362 (qval < 1E-05)

Base call quality data from DBCLS SRA