The cells were cross-linked with 1.5 mM EGS (Thermo Scientific) for 20 min, followed by treatment with 1% formaldehyde for 10 min; the samples were then lysed and digested with micrococcal nuclease (New England Biolabs) to shear DNA.ChIP was performed with anti-NRF1 antibody (Cell Signaling, D5B10).The antibody incubation were pereformed overnight at 4℃. The DNA libraries were prepared from 1 ng of ChIP and input samples quantified with Qubit Fluorometer (Life Technologies), using Mondrian SP+ and Ovation SP Ultralow DR Multiplex System (TaKaRa).braries were prepared for sequencing using standard Illumina protocols.