Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Blood
Cell type
P493-6
NA
NA

Attributes by original data submitter

Sample

source_name
B-cell lymphoma cell line
cell type
P493-6
chip antibody
c-Myc N262 (Santa Cruz , catalog# sc764, lot#G0111)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 250 bp were immunoprecipitated using different antibodies. Libraries were prepared for Illumina sequencing using a standard protocol consisting in blunting, addition of dA overhangs, ligation of Illumina adapters, selection on gel and PCR with index primers.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
23081441
Reads aligned (%)
45.6
Duplicates removed (%)
22.9
Number of peaks
980 (qval < 1E-05)

hg38

Number of total reads
23081441
Reads aligned (%)
47.1
Duplicates removed (%)
21.3
Number of peaks
618 (qval < 1E-05)

Base call quality data from DBCLS SRA