Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Prostate

Cell type

C4-2

Tissue

Prostate

Cell Type

Epithelial

Disease

Prostate Cancer

Sample

source_name

Human prostate cancer cell line C42B

tissue

Human prostate cancer cell line C42B

cell line

C42B

chip antibody

H3K4me3

sirna

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, cells were crosslinked using 1% formaldehyde andl lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq: Libraries were barcoded (NEXTflex™ DNA Barcodes) (Bio Scientific, Austin, TX).

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

55885234

Reads aligned (%)

99.0

Duplicates removed (%)

8.0

Number of peaks

1494 (qval < 1E-05)

hg19

Number of total reads

55885234

Reads aligned (%)

98.0

Duplicates removed (%)

9.6

Number of peaks

1336 (qval < 1E-05)