Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZFX

Cell type

Cell type Class
Prostate
Cell type
PrEC
Tissue
prostate
Lineage
epithelial
Description
prostate epithelial cell line

Attributes by original data submitter

Sample

source_name
Human normal prostate epithelial cell PrEC
tissue
Human normal prostate epithelial cell PrEC
cell line
PrEC
chip antibody
ZFX
sirna
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were crosslinked using 1% formaldehyde andl lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq: Libraries were barcoded (NEXTflex™ DNA Barcodes) (Bio Scientific, Austin, TX).

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg19

Number of total reads
26807088
Reads aligned (%)
77.0
Duplicates removed (%)
20.2
Number of peaks
3484 (qval < 1E-05)

hg38

Number of total reads
26807088
Reads aligned (%)
79.0
Duplicates removed (%)
18.8
Number of peaks
3599 (qval < 1E-05)

Base call quality data from DBCLS SRA