GSM1233298: NB4 MC2392 48h ChIP Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9K14ac
Cell type
Cell type Class
Blood
Cell type
NB-4
Primary Tissue
Blood
Site of Extraction
Bone Marrow
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
MC2392 treated NB4 cells, 48h ChIP
cell type
NB4 promyelocytic leukaemia cell line
treatment
MC2392 48h
chip antibody
H3K9K14ac (Diagenode)
reference genome
HG18
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sonicated and used for ChIP-seq using H3K9K14ac (Diagenode). RNA was extracted using qiagen RNeasy kit according to manufacturer. cDNA first and Ds-cDNA synthesis then, was performed following supplier’s instructions (Invitrogen). Ds-cDNA samples were prepared for sequencing by end repair of 20 ng DNA as measured by Qubit (Invitrogen). Adaptors were ligated to DNA fragments, followed by size selection (~300 bp) and limited PCR amplification (14 cycles). The resulting sample libraries were subjected to DSN treatment (Evrogen) according to the manufacturer’s recommendations. ChIP and RNA seq libraries were preapared according to Illumina's standard protocol.