Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Germinal center B-cells

NA

NA

Sample

source_name

Germinal Center

cell type

Sorted Germinal Center B cells

strain

C57BL/6

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27me3- (C36B11, Cell Signaling) or H3K4me3- (04-745, Millipore) specific antibodies. DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

90668647

Reads aligned (%)

95.2

Duplicates removed (%)

44.9

Number of peaks

901 (qval < 1E-05)

mm9

Number of total reads

90668647

Reads aligned (%)

95.0

Duplicates removed (%)

44.8

Number of peaks

967 (qval < 1E-05)