Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Th17 Cells

MeSH Description

A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Sample

source_name

Th17 cells cultured in vitro (TGFβ plus IL-6)

cell type

Th17 cells

strain

C57BL/6

genotype

TRIM28fl/fl

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared following the protocol of Active Motif's ChIP-IT Express Enzymatic Kit (#53035) and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Bioo Scientific's instructions accompanying the NEXTflex Illumina ChIP-Seq Library Prep Kit (#5143).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

16763533

Reads aligned (%)

99.4

Duplicates removed (%)

24.4

Number of peaks

279 (qval < 1E-05)

mm9

Number of total reads

16763533

Reads aligned (%)

99.3

Duplicates removed (%)

24.5

Number of peaks

275 (qval < 1E-05)