Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Embryos

NA

NA

Sample

source_name

embryo_ChIPSeq_input

genotype

5905

age

30min - 1 hour embryo

tissue

embryo

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-Seq, 5% mouse epigenome was used as internal control for H3K27me3 IP. ChIP libraries were prepared according to instructions of NEB DNA library prep kit (NEB, E7370L). RNA libraries were prepared according to instructions of NEB Ultra RNA library prep kit (NEB, E7530L).

Sequencing Platform

instrument_model

NextSeq 550

dm6

Number of total reads

10879524

Reads aligned (%)

90.2

Duplicates removed (%)

7.0

Number of peaks

1661 (qval < 1E-05)

dm3

Number of total reads

10879524

Reads aligned (%)

90.6

Duplicates removed (%)

4.7

Number of peaks

663 (qval < 1E-05)