GSM2902642: ChIP-seq for NFIC; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NFIC
Cell type
Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular
Attributes by original data submitter
Sample
source_name
HepG2 Cell line (ATCC® HB-8065™)
cell line
HepG2
antibody
NFIC
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The chromatin was decross-linked at 65 oC over night in TE buffer with 1% SDS, 500 mM NaCl, 20 ug of Proteinase K. DNA was extracted and purified with Qiagen MinElute kit. After reverse crosslinking, ChIP and input DNA fragments were used for library construction with NEBNext Ultra Ligation Module (E7445, NEB)