Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Pan-acetyllysine

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
Cultured cells
cell line
HepG2
chip antibody
Anti-Pan Kac (manufacturer: PTM Biolabs; catalog number: PTM-101; lot number: 01BC08M02)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared by MNase digestion and isolated with antibody. ChIP-seq libraries were prepared following the TruSEq Chip-SEQ kit (Illumina, San Diego, CA) as per manufacturer's instruction.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
48399990
Reads aligned (%)
99.3
Duplicates removed (%)
21.1
Number of peaks
11329 (qval < 1E-05)

hg19

Number of total reads
48399990
Reads aligned (%)
98.9
Duplicates removed (%)
22.0
Number of peaks
11216 (qval < 1E-05)

Base call quality data from DBCLS SRA