Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Embryo
Cell type
Blastoderm

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Blastoderm embryos
developmental stage
Blastoderm
strains
Oregon R, MV2-25, V46
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated by CsCl gradient ultracentrifugation, fragmented and immunoprecipitated with affnity-purified antibody Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly DNA fragments were converted to phosphorylated blunt ends using T4 DNA polymerase, Klenow DNA polymerase, and T4 polymerase kinase, a 3' A base overhang was added using Klenow DNA polymerase exo- (3' to 5' exo minus), and Illumina adapters were ligated to the fragments. We carried out the PCR step for enrichment of adapter-modified DNA prior to the library size selection, and limited the amplification to 15 cycles. After the amplification step, we size-selected DNA fragments of 150-250 bp.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
47987333
Reads aligned (%)
22.2
Duplicates removed (%)
19.1
Number of peaks
3908 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA