GSM2891897: Daunorubicin yH2AX; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H2A.XS139ph
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
K562_Daunorubicin
cell line
K562
treated with
Daunorubicin (10uM) for 4hrs
chip antibody
yH2AX (Millipore, 05-636, clone JBW301)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP: Cells were lysed, nuclei were isolated and sonicated, yH2AX bound DNA was isolated with antibody. For FAIRE: Cells were lysed, nuclei were isolated and sonicated, protein free DNA was isolated by phenol/Chlorophorm extraction. DNA libraries were prepared using standard protocols for sequencing on the Illumina HiSeq2000 platform.