Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H2A.XS139ph

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562_Daunorubicin
cell line
K562
treated with
Daunorubicin (10uM) for 4hrs
chip antibody
yH2AX (Millipore, 05-636, clone JBW301)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP: Cells were lysed, nuclei were isolated and sonicated, yH2AX bound DNA was isolated with antibody. For FAIRE: Cells were lysed, nuclei were isolated and sonicated, protein free DNA was isolated by phenol/Chlorophorm extraction. DNA libraries were prepared using standard protocols for sequencing on the Illumina HiSeq2000 platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
64459278
Reads aligned (%)
94.5
Duplicates removed (%)
2.9
Number of peaks
1687 (qval < 1E-05)

hg19

Number of total reads
64459278
Reads aligned (%)
93.8
Duplicates removed (%)
4.1
Number of peaks
1535 (qval < 1E-05)

Base call quality data from DBCLS SRA