GSM1226520: UNG KO gammaH2AX ChIP-seq, pemetrexed-treated; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H2A.XS139ph
Cell type
Cell type Class
Digestive tract
Cell type
DLD-1
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Cultured cells
cell line
DLD1
genotype/variation
UNG knockout; UNG-/-
treated with
pemetrexed
chip antibody
Anti-gamma H2A.X (phospho S139) antibody
chip antibody vendor
Abcam
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described (PMID 19275939). Sequencing libraries were constructed as described (PMID 19275939) with the exception of the library amplification step. Instead of Phusion DNA polymerase, Expand High Fidelity PLUS (Roche) was used with the following cycling parameters: The following parameters were used for thermal cycling: 5 min at 94°C, 18 cycles of 30 sec at 94°C, 30 sec at 65°C, and 60 sec at 72°C followed by a 7 min final elongation at 72°C.