Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SUZ12

Cell type

Cell type Class
Others
Cell type
Fibroblasts
NA
NA

Attributes by original data submitter

Sample

source_name
CRL7250 (fibroblast)
cell line
CRL7250 (fibroblast)
shRNA
SS18
antibody
anti-SUZ12
antibody vendor
Cell Signaling
antibody catalog
D39F6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
41483602
Reads aligned (%)
64.9
Duplicates removed (%)
32.0
Number of peaks
5720 (qval < 1E-05)

hg38

Number of total reads
41483602
Reads aligned (%)
66.3
Duplicates removed (%)
30.9
Number of peaks
5837 (qval < 1E-05)

Base call quality data from DBCLS SRA