Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
SYO1
NA
NA

Attributes by original data submitter

Sample

source_name
SYO1 (SS)
cell line
SYO1 (SS)
shRNA
shSSX
antibody
none, input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
35675629
Reads aligned (%)
98.1
Duplicates removed (%)
4.5
Number of peaks
959 (qval < 1E-05)

hg19

Number of total reads
35675629
Reads aligned (%)
97.3
Duplicates removed (%)
5.7
Number of peaks
902 (qval < 1E-05)

Base call quality data from DBCLS SRA