Antigen

Antigen Class

TFs and others

Antigen

SS18

Cell type

Cell type Class

Muscle

Cell type

SYO1

NA

NA

Sample

source_name

SYO1 (SS)

cell line

SYO1 (SS)

shRNA

shCt

antibody

anti-SS18

antibody vendor

Cell Signaling

antibody catalog

D6I4Z

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

27366438

Reads aligned (%)

97.4

Duplicates removed (%)

7.3

Number of peaks

2929 (qval < 1E-05)

hg19

Number of total reads

27366438

Reads aligned (%)

96.7

Duplicates removed (%)

8.6

Number of peaks

2893 (qval < 1E-05)