Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Others

Cell type

Aska

NA

NA

Sample

source_name

Aska-BAF47KO1 (SS)

cell line

Aska-BAF47KO1 (SS)

shRNA

shSSX

antibody

none, input

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

24130419

Reads aligned (%)

96.3

Duplicates removed (%)

5.8

Number of peaks

361 (qval < 1E-05)

hg19

Number of total reads

24130419

Reads aligned (%)

95.5

Duplicates removed (%)

6.7

Number of peaks

321 (qval < 1E-05)