GSM2887570: Aska shSSX H3K4me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Others
Cell type
Aska
NA
NA
Attributes by original data submitter
Sample
source_name
Aska (SS)
cell line
Aska (SS)
shRNA
shSSX
antibody
anti-H3K4me3
antibody vendor
Millipore
antibody catalog
05-745R
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500