Antigen

Antigen Class

TFs and others

Antigen

SS18

Cell type

Cell type Class

Others

Cell type

Aska

NA

NA

Sample

source_name

Aska (SS)

cell line

Aska (SS)

shRNA

shCt

antibody

anti-SS18

antibody vendor

Cell Signaling

antibody catalog

D6I4Z

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

27857683

Reads aligned (%)

97.8

Duplicates removed (%)

19.5

Number of peaks

3820 (qval < 1E-05)

hg19

Number of total reads

27857683

Reads aligned (%)

97.2

Duplicates removed (%)

20.7

Number of peaks

3800 (qval < 1E-05)