ChIP-Atlas: SRX3465815

Antigen

Cell type Class: Blood
Cell type: Macrophages
MeSH Description: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Formaldehyde crosslinked chromatin was sonicated to fragments DNA to about 200-300 bp, the immunoprecipitated with anti-NFAT5 antibodies. Immunoprecipitated chromatin was purified and sequenced. Chromatin aliquots from the wild-type and NFAT5-deficient BMDMs sample were separated to use as a measure of chromatin input. Libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ref. E7370) according to the manufacturer's protocol from 5-10 ng of starting material. Final libraries were analyzed using the Agilent Bioanalyzer with a DNA High Sensitivity chip to estimate the quantity and check size distribution, and were then quantified by qPCR using the KAPA Library Quantification Kit (KapaBiosystems, Ref. KK4835) prior to sequencing with Illumina HiSeq 2000