Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rela

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
bone marrow-derived macrophages (BMDM)
antibody
p65 - Santa Cruz, SC-372 (C-20), A2298
genotype/variation
wild type
treatment
control
cell type
bone marrow-derived macrophages (BMDM)
passages
1
strain
C57BL/6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP experiments, cell lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with LSD1 and p65 antibodies. ChIP-seq libraries were prepared for sequencing using the KAPA library preparation kit, according to the manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
29011313
Reads aligned (%)
1.4
Duplicates removed (%)
36.8
Number of peaks
68 (qval < 1E-05)

mm9

Number of total reads
29011313
Reads aligned (%)
1.4
Duplicates removed (%)
37.8
Number of peaks
56 (qval < 1E-05)

Base call quality data from DBCLS SRA