ChIP-Atlas: SRX3459536

Antigen

Cell type Class: Liver
Cell type: Liver
MeSH Description: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

source_name: liver
genotype/variation: Tetracycline-inducible YAPS127A
sample type: strain described in Camargo et al Curr Biol. 2007 Dec 4;17(23):2054-60
antibody: none

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Cells were cross-linked in 1% formaldehyde for 10 min at room temperature after which the reaction was stopped by addition of 0.125M glycine. Cells were lysed and harvested in ChIP buffer (100 mM Tris at pH 8.6, 0.3% SDS, 1.7% Triton X-100, and 5 mM EDTA) and the chromatin disrupted by sonication using a Diagenode Bioruptor sonicator UCD-200 to obtain fragments of average 200-500 bp in size. Suitable amounts of chromatin were incubated with specific antibodies overnight. Immunoprecipitated complexes were recovered on Protein-A/G agarose beads (Pierce) and, after extensive washes, DNA was recovered by reverse crosslinking and purification using QIAquick PCR purification kit (QIAGEN). Libraries for ChIP-sequencing were generated by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) and barcoded added using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB) according to manufacturer's recommendation.