Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
THP-1 24h after 1,25D treatment, 2nd replicate H3K27ac
cell type
THP-1
treatment protocol
24h with 1,25(OH)2D3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown in culture as mentioned above, after which they were crosslinked with formaldehyde, lysed and the DNA was extracted. Then, ChIP was performed ChIP DNA was sent to EMBL and the library was prepared there according to their protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
34434710
Reads aligned (%)
99.2
Duplicates removed (%)
16.5
Number of peaks
38939 (qval < 1E-05)

hg19

Number of total reads
34434710
Reads aligned (%)
98.8
Duplicates removed (%)
16.9
Number of peaks
38983 (qval < 1E-05)

Base call quality data from DBCLS SRA