Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4K12ac

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7
cell line
MCF7
experiment number
SLX-15090
cell type
mammary epithelial cells
chip antibody
H4K12ac (07-595,Millipore)
control antibody
CTCF (3418 XP, Cell Signaling)
treatment
Estrogen
replicate
2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to the Thruplex DNA-seq Kit Manual

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
86596638
Reads aligned (%)
99.3
Duplicates removed (%)
32.6
Number of peaks
82530 (qval < 1E-05)

hg19

Number of total reads
86596638
Reads aligned (%)
98.8
Duplicates removed (%)
33.0
Number of peaks
82662 (qval < 1E-05)

Base call quality data from DBCLS SRA