Antigen

Antigen Class

Histone

Antigen

H4K12ac

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7

cell line

MCF7

experiment number

SLX-15090

cell type

mammary epithelial cells

chip antibody

H4K12ac (07-595,Millipore)

control antibody

CTCF (3418 XP, Cell Signaling)

treatment

none

replicate

Input

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to the Thruplex DNA-seq Kit Manual

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

100853640

Reads aligned (%)

99.0

Duplicates removed (%)

13.7

Number of peaks

414 (qval < 1E-05)

hg19

Number of total reads

100853640

Reads aligned (%)

98.2

Duplicates removed (%)

14.0

Number of peaks

376 (qval < 1E-05)