Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUVBL2

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
cell type
liver cancer cell line
cell line
HepG2
antibody
RUVBL2
vendor
bethyl
catalog number
A302-537A
lot
A302-536A-T-1
treatment
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 0.3% formaldehyde for 30 minutes at 4 degrees prior to processing for ChIP using protocol from Raab et al 2015. Kapa Hyperprep kit using Illumina Truseq barcoded adapters

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
44787426
Reads aligned (%)
97.4
Duplicates removed (%)
0.6
Number of peaks
524 (qval < 1E-05)

hg19

Number of total reads
44787426
Reads aligned (%)
96.7
Duplicates removed (%)
0.6
Number of peaks
676 (qval < 1E-05)

Base call quality data from DBCLS SRA