Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MCRS1

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
cell type
liver cancer cell line
cell line
HepG2
antibody
MCRS1
vendor
protein-tech
catalog number
11362-1-AP
lot
1
treatment
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 0.3% formaldehyde for 30 minutes at 4 degrees prior to processing for ChIP using protocol from Raab et al 2015. Kapa Hyperprep kit using Illumina Truseq barcoded adapters

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
44580810
Reads aligned (%)
96.6
Duplicates removed (%)
0.5
Number of peaks
1139 (qval < 1E-05)

hg38

Number of total reads
44580810
Reads aligned (%)
97.3
Duplicates removed (%)
0.5
Number of peaks
1576 (qval < 1E-05)

Base call quality data from DBCLS SRA