Antigen

Antigen Class

TFs and others

Antigen

TAL1

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

Human K562 cells

cell line

K562

genotype/variation

wildtype

chip antibody

Santa Cruz Biotechnology,sc-12984,C1315

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq analysis using the Illumina NextSeq500, 1-10 ng of ChIP DNA was processed for library generation using the NEBNext Ultra II DNA Library Prep kit following the manufacturer's protocol (New England Biolabs). Raw reads that aligned to exactly one location in the reference human genome (hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

10867176

Reads aligned (%)

96.7

Duplicates removed (%)

22.8

Number of peaks

18434 (qval < 1E-05)

hg38

Number of total reads

10867176

Reads aligned (%)

97.3

Duplicates removed (%)

22.1

Number of peaks

18466 (qval < 1E-05)