Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562 cell line
cell line
K562
antibody
G4

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated from K562 cells that were cross-linked using 1% formaldehyde for 10 min at RT and prepared using a ChIP-qPCR Kit (Chromatrap, cat. no. 500117). Chromatin was sonicated using a Bioruptor Plus (Diagenode) to give a fragment distribution of 100-500 bp. G4 ChIPed DNA was subjected to Nextera library preparation (Illumina, cat. no. FC-121-1030). For further details see methods section in the associated manuscript.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
102690859
Reads aligned (%)
80.7
Duplicates removed (%)
33.3
Number of peaks
7597 (qval < 1E-05)

hg19

Number of total reads
102690859
Reads aligned (%)
80.1
Duplicates removed (%)
34.3
Number of peaks
7278 (qval < 1E-05)

Base call quality data from DBCLS SRA