Antigen

Antigen Class

TFs and others

Antigen

Cebpa

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

CEBPa from macrophages cells

strain background

C57Bl/6

cell type

bone Marrow derived Macrophages

chip antibody

C/EBPalpha

chip antibody vendor

Santa Cruz biotechnology

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Primary macrophages were differentiated from isolated bone marrow in IMDM medium supplemented with IL-3 and CSF1 and ChIP assays were performed with rabbit antibodies against C/EBPa (sc-61X, Santa Cruz). DNA from in vivo treated cells was run on an agarose gel and fragments in the range of 100-600 bp were excised and isolated. This DNA was used to prepare the libraries, according to Illumina's sample preparation protocol

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

13857158

Reads aligned (%)

65.6

Duplicates removed (%)

17.1

Number of peaks

32587 (qval < 1E-05)

mm9

Number of total reads

13857158

Reads aligned (%)

65.5

Duplicates removed (%)

17.3

Number of peaks

32585 (qval < 1E-05)